Media Bhi. BHI Agar is an enriched nonselective media used for the isolation and cultivation of a wide variety of bacteria including yeast and molds The basic nutritive properties of this media are brain heart infusion from solids meat peptone and yeast extract Dextrose provides a carbohydrate source for the fermentative microorganisms.
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PDF fileBHI Broth is also the preferred medium for anaerobic bacteria yeasts and moulds (24) This medium is nutritious and well buffered to support the growth of wide variety of organisms (256) With the addition of 10% defibrinated sheep blood it is useful for isolation and cultivation of Histoplasma capsulatum (7) and other fungi.
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Readytouse the convenience of prepared media BHI Agar contains brain heart infusion peptone and dextrose which provide nitrogen carbon sulfur vitamins and a carbohydrate source Sheep blood provides essential growth factors for fastidious fungi Yeast extracts are incorporated in the medium to serve as a growth stimulant.
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Brain Heart Infusion (BHI) Agar Composition, Principle
Brain heart infusion (BHI) is a growth medium for growing microorganisms It is a nutrientrich medium and can therefore be used to culture a variety of fastidious organisms In particular it has been used to culture streptococci pneumococci and meningococci which can be otherwise challenging to grow.
